Research area

Current projects:

• The structural and dynamic studies of the anticodon stem loop (ACSL) of tRNAPhe from E.coli (y32,y39-ACSL and i6A37y32y39-ACSL).
• Chemical synthesis of the site specific 15N-labeled nucleosides used in studies of the mechanism reaction of ACSL (tRNAPhe) with the isopentenyl transferase (MiaA).
• Enzymatic synthesis of pseudouridine containing 13C,15N-labeled RNAs using pseudouridine synthase (RluA).
• Determination of the metal binding sites in the complex of thio-modified Helix21 rRNA from E.coli with S8 ribosomal protein
• Structural studies of the snoRNA using NMR

Completed projects

• Synthesis of the modified and diastereomerically pure dinucleotides.
• Studies of the new phosphitylating reagents used in phosphoramidite synthesis of nucleotides.
• Studies of the reactivity and stability of P-F containing dinucleotides and their synthesis on the solid phase.